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验证数据展示

  • WB analysis using 67777-1-Ig (same clone as 67777-1-PBS)

    WB analysis using 67777-1-Ig (same clone as 67777-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67777-1-Ig (GOLPH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    WB analysis of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    WB result of GOLPH3 antibody (67777-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLPH3 transfected HeLa cells. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    IF Staining of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLPH3 antibody (67777-1-Ig, Clone: 3H2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GOLPH3 Monoclonal antibody, PBS Only

GOLPH3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67777-1-PBS

产品说明书

CloneNo. 3H2A5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MIDAS, GPP34, GOPP1, GOLPH 3, Golgi phosphoprotein 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67777-1-PBS targets GOLPH3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5443

Product name: Recombinant human GOLPH3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-298 aa of BC033725

Sequence: MTSLTQRSSGLVQRRTEASRNAADKERAAGGGAGSSEDDAQSRRDEQDDDDKGDSKETRLTLMEEVLLLGLKDREGYTSFWNDCISSGLRGCMLIELALRGRLQLEACGMRRKSLLTRKVICKSDAPTGDVLLDEALKHVKETQPPETVQNWIELLSGETWNPLKLHYQLRNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLTNNNIKQRLIKKVQEAVLDKWVNDPHRMDRRLLALIYLAHASDVLENAFAPLLDEQYDLATKRVRQLLDLDPEVECLKANTNEVLWAVVAAFTK

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 golgi phosphoprotein 3 (coat-protein)
别名 MIDAS, GPP34, GOPP1, GOLPH 3, Golgi phosphoprotein 3
计算分子量 298 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC033725
基因名称 GOLPH3
Gene ID (NCBI) 64083
RRIDAB_2918542
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H4A6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signaling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).

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