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验证数据展示

  • WB analysis of HeLa using 16090-1-AP

    WB analysis of HeLa using 16090-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 16090-1-AP

    IHC staining of human ovary tumor using 16090-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 16090-1-AP

    IHC staining of human ovary tumor using 16090-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16090-1-AP

    IF Staining of HepG2 using 16090-1-AP

    Immunofluorescent analysis of HepG2 cells, using GNB2 antibody 16090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HeLa using 16090-1-AP

    IF Staining of HeLa using 16090-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNB2 antibody (16090-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GNB2 Polyclonal antibody

GNB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16090-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16090-1-AP targets GNB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag9125

Product name: Recombinant human GNB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-340 aa of BC010073

Sequence: MSELEQLRQEAEQLRNQIRDARKACGDSTLTQITAGLDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPDGRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLLLAGYDDFNCNIWDAMKGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 2
别名 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2
计算分子量 340 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC010073
基因名称 GNB2
Gene ID (NCBI) 2783
RRIDAB_2111939
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62879
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNB2(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2) is also named as G protein subunit beta-2, transducin beta chain 2 and belongs to the WD repeat G protein beta family. G proteins are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

实验方案

Product Specific Protocols
IF protocol for GNB2 antibody 16090-1-APDownload protocol
IHC protocol for GNB2 antibody 16090-1-APDownload protocol
WB protocol for GNB2 antibody 16090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Front Cardiovasc Med

Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.

Authors - Yue Zheng
View Article
human,mouseWB

PLoS Biol

KCTD proteins regulate morphine dependence via heterologous sensitization of adenylyl cyclase 1 in mice

Authors - Zhong Ding
View Article
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