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GNB1 Polyclonal antibody

GNB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10247-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, GBB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10247-2-AP targets GNB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 GNB1 fusion protein Ag0313 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 1
别名 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, GBB1
计算分子量 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC004186
基因名称 GNB1
Gene ID (NCBI) 2782
RRIDAB_2111820
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62873
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).

实验方案

Product Specific Protocols
WB protocol for GNB1 antibody 10247-2-APDownload protocol
IHC protocol for GNB1 antibody 10247-2-APDownload protocol
IF protocol for GNB1 antibody 10247-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP

Cell

Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.

Authors - Gonzalo P Solis
  • KD Validated
humanIF,IP

PLoS Pathog

H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans.

Authors - Fangtao Li
mouseWB

Neuropharmacology.

A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.

Authors - Saba LM
mouseIHC

Exp Eye Res

Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction.

Authors - Haider Neena B NB
mouseWB

Dev Dyn

Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice.

Authors - Okae Hiroaki H
humanWB

Bioengineered

Shikonin induces cell autophagy via modulating the microRNA -545-3p/guanine nucleotide binding protein beta polypeptide 1 axis, thereby disrupting cellular carcinogenesis in colon cancer.

Authors - ZhiWei Hu
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