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CoraLite®594-conjugated GMNN Monoclonal antibody

GMNN Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E3A2

Cat No : CL594-66566

Print datasheet

Synonyms

Gem, Geminin, GMNN, RP3 369A17.3

验证数据展示

  • IF Staining of HeLa using CL594-66566

    IF Staining of HeLa using CL594-66566

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GMNN antibody (CL594-66566, Clone: 2E3A2 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66566-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66566 targets GMNN in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen GMNN fusion protein Ag24283 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name geminin, DNA replication inhibitor
Synonyms Gem, Geminin, GMNN, RP3 369A17.3
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC005185
Gene Symbol GMNN
Gene ID (NCBI) 51053
RRIDAB_2920007
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75496
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

实验方案

Product Specific Protocols
IF protocol for CL594 GMNN antibody CL594-66566Download protocol
Standard Protocols
Click here to view our Standard Protocols