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验证数据展示

  • WB analysis using 86101-2-RR

    WB analysis using 86101-2-RR

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 86101-2-RR (GMIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 86101-2-RR

    IHC staining of human ovary cancer using 86101-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86101-2-RR (GMIP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GMIP antibody included
  • IF Staining of K-562 using 86101-2-RR

    IF Staining of K-562 using 86101-2-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GMIP antibody (86101-2-RR, Clone: 250483G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86101-2-RR

    Affinity and Kinetic Characterization of 86101-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86101-2-RR against Human GMIP were performed. The affinity constant is 2.02 nM.

GMIP Recombinant monoclonal antibody

GMIP Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86101-2-RR

产品说明书

CloneNo. 250483G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ARHGAP46, GEM interacting protein, GEM-interacting protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy® GMIP Ready-To-Use IHC Kit. GMIP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86101-2-RR targets GMIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23239

Product name: Recombinant human GMIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC126436

Sequence: MDAAEPGLPPGPEGRKRYSDIFRSLDNLEISLGNVTLEMLAGDPLLSEDPEPDKTPTATVTNEASCWSGPSPEGPVPLTGEELDLRLIRTK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GEM interacting protein
别名 ARHGAP46, GEM interacting protein, GEM-interacting protein
观测分子量 130 kDa
GenBank蛋白编号BC126436
基因名称 GMIP
Gene ID (NCBI) 51291
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P107
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.

实验方案

Product Specific Protocols
IF protocol for GMIP antibody 86101-2-RRDownload protocol
IHC protocol for GMIP antibody 86101-2-RRDownload protocol
WB protocol for GMIP antibody 86101-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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