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验证数据展示

  • WB analysis using 86101-2-RR (same clone as 86101-2-PBS)

    WB analysis using 86101-2-RR (same clone as 86101-2-PBS)

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 86101-2-RR (GMIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 86101-2-RR (same clone as 86101-2-PBS)

    IHC staining of human ovary cancer using 86101-2-RR (same clone as 86101-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86101-2-RR (GMIP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GMIP antibody included
  • IF Staining of K-562 using 86101-2-RR (same clone as 86101-2-PBS)

    IF Staining of K-562 using 86101-2-RR (same clone as 86101-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GMIP antibody (86101-2-RR, Clone: 250483G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86101-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86101-2-RR

    Affinity and Kinetic Characterization of 86101-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86101-2-RR against Human GMIP were performed. The affinity constant is 2.02 nM.

GMIP Recombinant monoclonal antibody, PBS Only

GMIP Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86101-2-PBS

产品说明书

CloneNo. 250483G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ARHGAP46, GEM interacting protein, GEM-interacting protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® GMIP Ready-To-Use IHC Kit. GMIP primary antibody included.

产品信息

86101-2-PBS targets GMIP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23239

Product name: Recombinant human GMIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC126436

Sequence: MDAAEPGLPPGPEGRKRYSDIFRSLDNLEISLGNVTLEMLAGDPLLSEDPEPDKTPTATVTNEASCWSGPSPEGPVPLTGEELDLRLIRTK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GEM interacting protein
别名 ARHGAP46, GEM interacting protein, GEM-interacting protein
观测分子量 130 kDa
GenBank蛋白编号BC126436
基因名称 GMIP
Gene ID (NCBI) 51291
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P107
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.

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