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验证数据展示

  • WB analysis of human placenta using 13314-1-AP

    WB analysis of human placenta using 13314-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 13314-1-AP

    IHC staining of human cervical cancer using 13314-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with GMEB2 antibody included
  • IHC staining of human cervical cancer using 13314-1-AP

    IHC staining of human cervical cancer using 13314-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with GMEB2 antibody included
  • IF Staining of MCF-7 using 13314-1-AP

    IF Staining of MCF-7 using 13314-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GMEB2 antibody (13314-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

GMEB2 Polyclonal antibody

GMEB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13314-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse ovary tissue, mouse thymus tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GMEB2 Ready-To-Use IHC Kit. GMEB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13314-1-AP targets GMEB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GMEB2 fusion protein Ag4157 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucocorticoid modulatory element binding protein 2
别名 P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2
计算分子量 530 aa, 56 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC036305
基因名称 GMEB2
Gene ID (NCBI) 26205
RRIDAB_2110829
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKD1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMEB2, also named as Glucocorticoid modulatory element-binding protein 2, is a 530 amino acid protein, which is expressed in peripheral blood lymphocytes and fetal liver. GMEB2 as a trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. The calcualted molecular weight of GMEB2 is 56 kDa, but modified GMEB2 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for GMEB2 antibody 13314-1-APDownload protocol
IHC protocol for GMEB2 antibody 13314-1-APDownload protocol
IF protocol for GMEB2 antibody 13314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols