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GMCL1 Polyclonal antibody

GMCL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15575-1-AP

Print datasheet

Synonyms

BTBD13, GCL, GCL1, Germ cell less protein like 1, GMCL1

验证数据展示

  • WB analysis using 15575-1-AP

    WB analysis using 15575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15575-1-AP (GMCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid using 15575-1-AP

    IHC staining of human thyroid using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

  • IHC staining of human thyroid using 15575-1-AP

    IHC staining of human thyroid using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman thyroid tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15575-1-AP targets GMCL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen GMCL1 fusion protein Ag7929 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name germ cell-less homolog 1 (Drosophila)
Synonyms BTBD13, GCL, GCL1, Germ cell less protein like 1, GMCL1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC007420
Gene Symbol GMCL1
Gene ID (NCBI) 64395
RRIDAB_2878153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMCL1, also named as GCL, functions in spermatogenesis. It enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport. In WB test, the antibody recognizes 55 kDa band in our detection.

实验方案

Product Specific Protocols
WB protocol for GMCL1 antibody 15575-1-APDownload protocol
IHC protocol for GMCL1 antibody 15575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols