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验证数据展示

  • IF Staining of HeLa using CL488-26456

    IF Staining of HeLa using CL488-26456

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GMAP-210 antibody (CL488-26456) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GMAP-210 Polyclonal antibody

GMAP-210 Polyclonal Antibody for IF/ICC
Cat No. CL488-26456

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

CEV14, GMAP 210, GMAP-210, TR interacting protein 11, TRIP 11, TRIP11, TRIP230

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIP11 Ready-To-Use IHC Kit. TRIP11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26456-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26456 targets GMAP-210 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 GMAP-210 fusion protein Ag13867 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thyroid hormone receptor interactor 11
别名 CEV14, GMAP 210, GMAP-210, TR interacting protein 11, TRIP 11, TRIP11, TRIP230
计算分子量 1979 aa, 228 kDa
观测分子量 230 kDa
GenBank蛋白编号BC002656
基因名称 TRIP11
Gene ID (NCBI) 9321
RRIDAB_3672798
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15643
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi microtubule-associated protein 210 (GMAP-210), also referred to as CEV14, Trip11 or Trip230, is a peripheral Golgi protein that localizes to the cis-Golgi network. GMAP-210 is a 1,978 amino acid coiled-coil member of the golgin family of proteins. Microtubule ends bind to GMAP-210 which functions to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. This interaction may be essential for the proper morphology and structural maintenance of the Golgi apparatus. GMAP-210 also associates with thyroid hormone receptor. Overexpression of GMAP-210 disrupts the micro-tubule network and causes a significant enlargement and fragmentation of the Golgi apparatus; it also blocks anterograde and retrograde transport between the ER and the Golgi apparatus.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GMAP-210 antibody CL488-26456Download protocol
Standard Protocols
Click here to view our Standard Protocols