验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue |
| Positive IHC detected in | human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
55292-1-AP targets ZGLP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GATA like protein-1 |
| 别名 | GATA like protein 1, GLP 1, GLP1, GLP-1, ZGLP1 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | NM_001103167 |
| 基因名称 | GLP-1 |
| Gene ID (NCBI) | 100125288 |
| RRID | AB_2881301 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0C6A0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZGLP1, also known as GLP-1, is a nuclear protein containing two zinc fingers, one of which is highly homologous to the conserved GATA zinc finger DNA binding motif. It is a transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZGLP1 antibody 55292-1-AP | Download protocol |
| WB protocol for ZGLP1 antibody 55292-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Chin J Integr Med Huanglian-Renshen-Decoction Maintains Islet β-Cell Identity in T2DM Mice through Regulating GLP-1 and GLP-1R in Both Islet and Intestine | ||
Front Pharmacol Berberine enhances the function of db/db mice islet β cell through GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and islet α cell |
