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验证数据展示

  • WB analysis of human milk using 16508-1-AP

    WB analysis of human milk using 16508-1-AP

    human milk were subjected to SDS PAGE followed by western blot with 16508-1-AP (GLIPR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 16508-1-AP

    IHC staining of human lung using 16508-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16508-1-AP (GLIPR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 16508-1-AP

    IF Staining of A431 using 16508-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GLIPR2 antibody (16508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GLIPR2 Polyclonal antibody

GLIPR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16508-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Glioma pathogenesis-related protein 2, GLI pathogenesis related 2, GAPR-1, GAPR1, GAPR 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman milk
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16508-1-AP targets GLIPR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GLIPR2 fusion protein Ag9725 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GLI pathogenesis-related 2
别名 Glioma pathogenesis-related protein 2, GLI pathogenesis related 2, GAPR-1, GAPR1, GAPR 1
计算分子量153aa,17 kDa; 90aa,10 kDa
观测分子量17 kDa
GenBank蛋白编号BC017918
基因名称 GLIPR2
Gene ID (NCBI) 152007
RRIDAB_3669246
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4G4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi-associated plant pathogenesis-related protein 1 (GLIPR2, also known as C9orf19 and GAPR1) is a multifunctional protein with dual engagement in normal cellular processes and the intricacies of cancer biology. GLIPR2 has also been associated with a spectrum of cellular functions encompassing the regulation of autophagy (PMID: 12137952; 18824526). It has been reported that GLIPR2 is a newly identified negative regulator of autophagy that inhibits the class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1) complex activity (PMID: 33222586).

实验方案

Product Specific Protocols
WB protocol for GLIPR2 antibody 16508-1-APDownload protocol
IHC protocol for GLIPR2 antibody 16508-1-APDownload protocol
IF protocol for GLIPR2 antibody 16508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols