×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 34250-1-AP (same clone as 34250-1-PBS)

    WB analysis using 34250-1-AP (same clone as 34250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 34250-1-AP (GLG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 34250-1-AP (GLG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 34250-1-AP (GLG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 34250-1-AP (same clone as 34250-1-PBS)

    FC experiment of HepG2 using 34250-1-AP (same clone as 34250-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.5 ug GLG1 Polyclonal antibody (34250-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

GLG1 Polyclonal antibody, PBS Only

GLG1 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 34250-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

CFR 1, CFR1, E selectin ligand 1, ESL 1, ESL1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

34250-1-PBS targets GLG1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag41864

Product name: Recombinant human GLG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 997-1146 aa of BC060822

Sequence: LDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi apparatus protein 1
别名 CFR 1, CFR1, E selectin ligand 1, ESL 1, ESL1
计算分子量 135 kDa
观测分子量 150 kDa
GenBank蛋白编号BC060822
基因名称 GLG1
Gene ID (NCBI) 2734
RRIDAB_3743180
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ92896
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgi apparatus protein 1 (GLG1) is also named CFR1, E-selectin ligand 1 (ESL-1) and MG160. GLG1 is a 150-160 kDa membrane sialoglycoprotein, originally isolated from the Golgi apparatus of rat neurons. Two homologs, the chicken cysteine-rich fibroblast growth factor receptor (CFR) and ESL-1, were identified in embryonic chick and murine myeloid cells, respectively (PMID: 35543162). GLG1 was originally identified as an FGF2 receptor, found in the Golgi complex (PMID:7531823). GLG1, a non-tyrosine kinase FGF receptor, is also regulated by RNA splicing (PMID: 7768993). GLG1 was found due to its significant upregulation in GC and correlation with advanced stages and lymph node metastases (PMID: 41287415).

||
New chat

Able™

正在加载，请稍候...