验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, SCaBER cells, SiHa cells, mouse colon tissue, rat colon tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.5 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34250-1-AP targets GLG1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag41864 Product name: Recombinant human GLG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 997-1146 aa of BC060822 Sequence: LDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi apparatus protein 1 |
| 别名 | CFR 1, CFR1, E selectin ligand 1, ESL 1, ESL1 |
| 计算分子量 | 135 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC060822 |
| 基因名称 | GLG1 |
| Gene ID (NCBI) | 2734 |
| RRID | AB_3743180 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q92896 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgi apparatus protein 1 (GLG1) is also named CFR1, E-selectin ligand 1 (ESL-1) and MG160. GLG1 is a 150-160 kDa membrane sialoglycoprotein, originally isolated from the Golgi apparatus of rat neurons. Two homologs, the chicken cysteine-rich fibroblast growth factor receptor (CFR) and ESL-1, were identified in embryonic chick and murine myeloid cells, respectively (PMID: 35543162). GLG1 was originally identified as an FGF2 receptor, found in the Golgi complex (PMID:7531823). GLG1, a non-tyrosine kinase FGF receptor, is also regulated by RNA splicing (PMID: 7768993). GLG1 was found due to its significant upregulation in GC and correlation with advanced stages and lymph node metastases (PMID: 41287415).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for GLG1 antibody 34250-1-AP | Download protocol |
| IHC protocol for GLG1 antibody 34250-1-AP | Download protocol |
| WB protocol for GLG1 antibody 34250-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
