验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse kidney tissue, mouse liver tissue, mouse testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13360-1-AP targets Glycerokinase in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag4175 Product name: Recombinant human GK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-357 aa of BC037549 Sequence: MAASKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILHSVYECIEKTCEKLGQLNIDISNIKAIGVSNQRETTVVWDKITGEPLYNAVVWLDLRTQSTVESLSKRIPGNNNFVKSKTGLPLSTYFSAVKLRWLLDNVRKVQKAVEEKRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKQLCEFFGIPMEILPNVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQIGQAKNTYGTGCFLLCNTGHKCVFSDHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKTSEEIEKLAKEVGTSYG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycerol kinase |
| 别名 | GK, GKD, GK1, EC:2.7.1.30, ATP:glycerol 3-phosphotransferase |
| 计算分子量 | 524 aa, 57 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC037549 |
| 基因名称 | Glycerokinase |
| Gene ID (NCBI) | 2710 |
| RRID | AB_1167391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P32189 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Glycerokinase antibody 13360-1-AP | Download protocol |
| IP protocol for Glycerokinase antibody 13360-1-AP | Download protocol |
| WB protocol for Glycerokinase antibody 13360-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis | ||
Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. | ||
J Appl Toxicol Aberrant expression of miR-451a contributes to 1,2-dichloroethane-induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice. | ||
Life Sci Alliance Serine palmitoyltransferase assembles at ER-mitochondria contact sites. | ||
J Adv Res O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation. | ||
Mol Metab The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326 |
