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验证数据展示

  • IHC staining of human oesophagus using 24215-1-AP

    IHC staining of human oesophagus using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human oesophagus using 24215-1-AP

    IHC staining of human oesophagus using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 40x lens).

GJB6 Polyclonal antibody

GJB6 Polyclonal Antibody for IHC, ELISA
Cat No. 24215-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

Connexin 30, CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, Gap junction beta 6 protein, GJB6, HED

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman oesophagus tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24215-1-AP targets GJB6 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21529

Product name: Recombinant human GJB6 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 182-261 aa of BC038934

Sequence: ISRPTEKTVFTIFMISASVICMLLNVAELCYLLLKVCFRRSKRAQTQKNHPNHALKESKQNEMNELISDSGQNAITGFPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gap junction protein, beta 6, 30kDa
别名 Connexin 30, CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, Gap junction beta 6 protein, GJB6, HED
计算分子量 261 aa, 30 kDa
GenBank蛋白编号BC038934
基因名称 GJB6
Gene ID (NCBI) 10804
RRIDAB_2879461
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO95452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gap junctions form conduits between adjacent cells that are composed of connexin protein subunits and provide direct intercellular communication pathways allowing rapid exchange of ions and metabolites (PMID: 12126230; 15094343). Connexins are four-pass transmembrane proteins with amino- and carboxy-terminal regions facing the cytoplasm. A connexon is composed of a hexamer of connexins. GJB6 (gap junction beta-6 protein, also known as connexin-30) is a member of the connexin family of proteins. GJB6 is a component of the gap junction networks of the cochlea.

实验方案

Product Specific Protocols
IHC protocol for GJB6 antibody 24215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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