验证数据展示
经过测试的应用
| Positive WB detected in | mouse stomach tissue, mouse ovary tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse placenta tissue, human ovary tumor tissue, human cervix tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12880-1-AP targets GJB3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, marmoset |
| 免疫原 | GJB3 fusion protein Ag3839 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gap junction protein, beta 3, 31kDa |
| 别名 | EKV, DFNA2B, DFNA2, CX31, Connexin-31 |
| 计算分子量 | 270 aa, 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC012918 |
| 基因名称 | GJB3 |
| Gene ID (NCBI) | 2707 |
| RRID | AB_2110907 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75712 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GJB3 antibody 12880-1-AP | Download protocol |
| IHC protocol for GJB3 antibody 12880-1-AP | Download protocol |
| IF protocol for GJB3 antibody 12880-1-AP | Download protocol |
| IP protocol for GJB3 antibody 12880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. | ||
Stem Cells FBXL12-Mediated Degradation of ALDH3 Is Essential for Trophoblast Differentiation during Placental Development. | ||
J Neurosci Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis. | ||
Sci Rep Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea. | ||
Placenta In vitro fertilization causes excessive glycogen accumulation in mouse placenta. | ||
