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验证数据展示

  • IHC staining of mouse heart using 15386-1-AP

    IHC staining of mouse heart using 15386-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with GJA1/Connexin 43 antibody included
  • IHC staining of mouse heart using 15386-1-AP

    IHC staining of mouse heart using 15386-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with GJA1/Connexin 43 antibody included
  • IF Staining of mouse heart using 15386-1-AP

    IF Staining of mouse heart using 15386-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HeLa using 15386-1-AP

    WB analysis of HeLa using 15386-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15386-1-AP (GJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 15386-1-AP

    IP experiment of mouse heart using 15386-1-AP

    IP result of anti-Connexin-43 (IP:15386-1-AP, 4ug; Detection:15386-1-AP 1:500) with mouse heart tissue lysate 3200ug.

  • IF Staining of HeLa using 15386-1-AP

    IF Staining of HeLa using 15386-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

Connexin-43 Polyclonal antibody

Connexin-43 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 15386-1-AP

产品说明书

引用文献(35)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

别名

GJA1, Connexin 43, GJAL, Gap junction alpha-1 protein, Gap junction alpha 1 protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GJA1/Connexin 43 Ready-To-Use IHC Kit. GJA1/Connexin 43 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15386-1-AP targets Connexin-43 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, bovine
免疫原

CatNo: Ag6698

Product name: Recombinant human Connexin 43 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-382 aa of BC026329

Sequence: MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gap junction protein, alpha 1, 43kDa
别名 GJA1, Connexin 43, GJAL, Gap junction alpha-1 protein, Gap junction alpha 1 protein
计算分子量 43 kDa
观测分子量 43-47 kDa
GenBank蛋白编号BC026329
基因名称 Connexin-43
Gene ID (NCBI) 2697
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17302
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Connexin-43 (Cx43, also known as gap junction alpha-1), a connexin family member, plays an essential role in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

实验方案

Product Specific Protocols
IF protocol for Connexin-43 antibody 15386-1-APDownload protocol
IHC protocol for Connexin-43 antibody 15386-1-APDownload protocol
IP protocol for Connexin-43 antibody 15386-1-APDownload protocol
WB protocol for Connexin-43 antibody 15386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.

Authors - Yin Wang
View Article
mouseIF

Thromb Res

Selective increase of cardiomyocyte derived extracellular vesicles after experimental myocardial infarction and functional effects on the endothelium.

Authors - Jose A Rodriguez
View Article
mouseWB

Hypertension

Novel Role for the Immunoproteasome Subunit PSMB10 in Angiotensin II-Induced Atrial Fibrillation in Mice.

Authors - Jing Li
View Article
IF

Ecotoxicol Environ Saf

Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MC-LR) via TLR4/NF-kB and mitochondrial signaling pathways blockage.

Authors - E O Adegoke
View Article
humanWB

Environ Toxicol Pharmacol

Microcystin-leucine arginine (MC-LR) induced inflammatory response in bovine sertoli cell via TLR4/NF-kB signaling pathway.

Authors - E O Adegoke
View Article
humanIF

Free Radic Biol Med

Oxidative stress contributes to hepatocyte growth factor-dependent pro-senescence activity of ovarian cancer cells.

Authors - Justyna Mikuła-Pietrasik
View Article
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