验证数据展示
经过测试的应用
| Positive WB detected in | ROS1728 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
26247-1-AP targets GIT1 in WB, IF, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag24648 Product name: Recombinant human GIT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 471-640 aa of BC067358 Sequence: LRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor kinase interacting ArfGAP 1 |
| 别名 | Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, CAT 1, ARF GTPase-activating protein GIT1 |
| 计算分子量 | 694 aa, 77 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC067358 |
| 基因名称 | GIT1 |
| Gene ID (NCBI) | 28964 |
| RRID | AB_2880445 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2X7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GIT1 (ARF GTPase-activating protein GIT1) is a member of the GIT subfamily of ADP ribosylation factor (ARF)-GTPase activating protein (GAP) family of proteins, which are characterized by an ARFGAP domain that stimulates the GTPase activity of proteins. GIT1 was highly expressed in the nervous system, and its expression was maintained throughout all brain neuritogenesis stages (PMID: 27127481).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GIT1 antibody 26247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Talin-tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation. | ||
Cancer Lett CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma. |
