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验证数据展示

  • WB analysis using 24790-1-AP (same clone as 24790-1-PBS)

    WB analysis using 24790-1-AP (same clone as 24790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 24790-1-AP (same clone as 24790-1-PBS)

    IP experiment of Jurkat using 24790-1-AP (same clone as 24790-1-PBS)

    IP result of anti-GIGYF2 (IP:24790-1-AP, 4ug; Detection:24790-1-AP 1:2000) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 24790-1-AP (same clone as 24790-1-PBS)

    IHC staining of rat brain using 24790-1-AP (same clone as 24790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GIGYF2 antibody included
  • IHC staining of mouse brain using 24790-1-AP (same clone as 24790-1-PBS)

    IHC staining of mouse brain using 24790-1-AP (same clone as 24790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GIGYF2 antibody included
  • IF Staining of HeLa using 24790-1-AP (same clone as 24790-1-PBS)

    IF Staining of HeLa using 24790-1-AP (same clone as 24790-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GIGYF2 antibody (24790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24790-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GIGYF2 Polyclonal antibody, PBS Only

GIGYF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24790-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

GRB10-interacting GYF protein 2, GYF2, KIAA0642, PERQ amino acid-rich with GYF domain-containing protein 2, PERQ2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GIGYF2 Ready-To-Use IHC Kit. GIGYF2 primary antibody included.

产品信息

24790-1-PBS targets GIGYF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20329

Product name: Recombinant human GIGYF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1104 aa of BC146775

Sequence: KRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRB10 interacting GYF protein 2
别名 GRB10-interacting GYF protein 2, GYF2, KIAA0642, PERQ amino acid-rich with GYF domain-containing protein 2, PERQ2
计算分子量 1320 aa, 152 kDa
观测分子量 150-170 kDa
GenBank蛋白编号BC146775
基因名称 GIGYF2
Gene ID (NCBI) 26058
RRIDAB_2879727
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6Y7W6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451).

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