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验证数据展示

  • WB analysis of U-87 MG using 24674-1-AP

    WB analysis of U-87 MG using 24674-1-AP

    WB result of GGT7 antibody (24674-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGT7 transfected U-87 MG cells.

  • WB analysis of HEK-293 using 24674-1-AP

    WB analysis of HEK-293 using 24674-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24674-1-AP (GGT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24674-1-AP

    WB analysis using 24674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24674-1-AP (GGT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 24674-1-AP

    IHC staining of human lung cancer using 24674-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24674-1-AP (GGT7 Antibody) at dilution of 1:50.

  • IHC staining of human lung cancer using 24674-1-AP

    IHC staining of human lung cancer using 24674-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24674-1-AP (GGT7 Antibody) at dilution of 1:50.

  • IHC staining of mouse brain using 24674-1-AP

    IHC staining of mouse brain using 24674-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24674-1-AP (GGT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 24674-1-AP

    IHC staining of rat brain using 24674-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24674-1-AP (GGT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • KD/KO Validated

GGT7 Polyclonal antibody

GGT7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24674-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (2)

别名

EC:2.3.2.2, EC:3.4.19.13, gamma glutamyltransferase 7, Gamma-glutamyltransferase 7, Gamma-glutamyltransferase-like 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, U-87 MG cells, hTERT-RPE1 cells
Positive IHC detected inmouse brain tissue, human lung tissue, human lung cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24674-1-AP targets GGT7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag20234

Product name: Recombinant human GGT7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 313-662 aa of BC141843

Sequence: VLDVLGTSGPAAFYAGGNLTLEMVAEAQHAGGVITEEDFSNYSALVEKPVCGVYRGHLVLSPPPPHTGPALISALNILEGFNLTSLVSREQALHWVAETLKIALALASRLGDPVYDSTITESMDDMLSKVEAAYLRGHINDSQAAPAPLLPVYELDGAPTAAQVLIMGPDDFIVAMVSSLNQPFGSGLITPSGILLNSQMLDFSWPNRTANHSAPSLENSVQPGKRPLSFLLPTVVRPAEGLCGTYLALGANGAARGLSGLTQVLLNVLTLNRNLSDSLARGRLHPDLQSNLLQVDSEFTEEEIEFLEARGHHVEKVDVLSWVHGSRRTNNFIIAVKDPRSPDAAGATIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gamma-glutamyltransferase 7
别名 EC:2.3.2.2, EC:3.4.19.13, gamma glutamyltransferase 7, Gamma-glutamyltransferase 7, Gamma-glutamyltransferase-like 3
计算分子量 662 aa, 70 kDa
观测分子量 75-100 kDa
GenBank蛋白编号BC141843
基因名称 GGT7
Gene ID (NCBI) 2686
RRIDAB_2879667
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJ14
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GGT7 (Glutathione hydrolase 7), a member of the gamma-glutamyltransferase family, is highly expressed in brain and was previously shown to have decreased expression in gliomas. Reports on the protein expression analysis of GGT7 are only limited to ovarian cancer and glioblastoma. Loss of GGT7 would lead to increased ROS activity, and in turn, stimulate tumorigenic phenotypes (PMID: 41316810, 25884624). GGT7 was identified as a heterodimer consisting of two glycosylated subunits with a mean molecular mass of 80 kDa and 29 kDa, respectively (PMID: 33916150).

实验方案

Product Specific Protocols
IHC protocol for GGT7 antibody 24674-1-APDownload protocol
WB protocol for GGT7 antibody 24674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF

iScience

Rapid fluorescent vital imaging of olfactory epithelium.

Authors - Hironobu Nishijima
View Article
humanIHC

bioRxiv

Perturbations of glutathione and sphingosine metabolites in Port Wine Birthmark patient-derived induced pluripotent stem cells

Authors - Vi Nguyen
View Article
humanIHC

Metabolites

Perturbations of Glutathione and Sphingosine Metabolites in Port Wine Birthmark Patient-Derived Induced Pluripotent Stem Cells

Authors - Vi Nguyen
View Article
mouse,humanWB,IP

Genome Biol

Systematic interrogation of functional genes underlying cholesterol and lipid homeostasis

Authors - Haihuan Shan
  • KO Validated
View Article
humanWB

Biochem Biophys Res Commun

Human PRSS8 functions as a targetable, endogenous TGFBR3 sheddase via the novel cholesterol-PRSS8-TGF-β axis.

Authors - Benjamin M Greulich
  • KD Validated
View Article
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