验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human placenta tissue, human testis tissue, mouse bladder tissue, human heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14944-1-AP targets GGPS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6745 Product name: Recombinant human GGPS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC067768 Sequence: MEKTQETVQRILLEPYKYLLQLPGKQVRTKLSQAFNHWLKVPEDKLQIIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAHSIYGIPSVINSANYVYFLGLEKVLTLDHQDAVKLFTRQLLELHQGQGLDIYWRDNYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLNTLGLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENIDIKKYCVHYLEDVGSFEYTRNTLKELEAKAYKQIDARGGNPELVALVKHLSKMFKEENE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | geranylgeranyl diphosphate synthase 1 |
| 别名 | GGPP synthase, GGPPS, GGPPS1, GGPPSase, GGPS1 |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC067768 |
| 基因名称 | GGPS1 |
| Gene ID (NCBI) | 9453 |
| RRID | AB_2110990 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GGPS1(Geranylgeranyl pyrophosphate synthase) belongs to the FPP/GGPP synthase family and catalyzes the synthesis of GGPP, an important molecule responsible for the C20-prenylation of protein and for the regulation of a nuclear hormone receptor. GGPP synthase puri¢ed from bovine brain is reported to be a homo-oligomer (150-195 kDa) with a molecular mass of 37.5 kDa for the monomer. This is consistent with the calculated molecular mass (35 kDa) of mouse and human GGPP synthases (PMID:10101267).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GGPS1 antibody 14944-1-AP | Download protocol |
| IP protocol for GGPS1 antibody 14944-1-AP | Download protocol |
| WB protocol for GGPS1 antibody 14944-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cancer Therapeutic targeting of the mevalonate-geranylgeranyl diphosphate pathway with statins overcomes chemotherapy resistance in small cell lung cancer.
| ||
Dev Cell Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development | ||
Int J Biol Macromol Targeting geranylgeranyl diphosphate synthase suppresses interleukin-1β-driven proliferation in lung squamous cell carcinoma by inhibiting Ras homolog family member A and Rac family small GTPase 1 geranylgeranylation. | ||
Cell Rep ENKD1 modulates innate immune responses through enhanced geranylgeranyl pyrophosphate synthase activity | ||
Cells Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model. | ||
Int Immunopharmacol Geranylgeranyl diphosphate synthase 1 knockdown suppresses NLRP3 inflammasome activity via promoting autophagy in sepsis-induced acute lung injury. |
