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GGH Polyclonal antibody

GGH Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13264-1-AP

Print datasheet

Synonyms

Conjugase, Gamma Glu X carboxypeptidase, Gamma glutamyl hydrolase, GGH, GH

验证数据展示

  • WB analysis using 13264-1-AP

    WB analysis using 13264-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 13264-1-AP

    IP experiment of HepG2 using 13264-1-AP

    IP result of anti-GGH (IP:13264-1-AP, 4ug; Detection:13264-1-AP 1:500) with HepG2 cells lysate 5600 ug.

  • IHC staining of human kidney using 13264-1-AP

    IHC staining of human kidney using 13264-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 13264-1-AP

    IHC staining of human kidney using 13264-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, MCF-7 cells, HepG2 cells, HT-1080 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13264-1-AP targets GGH in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GGH fusion protein Ag3939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
Synonyms Conjugase, Gamma Glu X carboxypeptidase, Gamma glutamyl hydrolase, GGH, GH
Calculated Molecular Weight 318 aa, 36 kDa
Observed Molecular Weight 30-37 kDa, 55 kDa
GenBank Accession NumberBC025025
Gene Symbol GGH
Gene ID (NCBI) 8836
RRIDAB_2877931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92820
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GGH (Gamma glutamyl hydrolase), also named as GH or Conjugase, is a key lysosomal enzyme involved in the metabolism of folic acid and in the action of antifolate drugs (PMID: 16945597). GGH catalyzes the removal of γ-linked polyglutamates from the intracellular folylpolyglutamates to yield folylmonoglutamate coenzymes (PMID: 9614206). The full-length protein has a calculated molecular mass of 36 kDa, contains four potential asparagine glycosylation sites, and was predicted to have a 24-amino-acid signal peptide (PMID: 8816764). GGH can form homodimer which contains two potential active sites (PMID: 16945597). Some bands can be detected by SDS-PAGE: 35-37kDa (full-length), 55kDa (glycosylated form) and 30-33kDa (signal peptide removed) (PMID: 8621474).

实验方案

Product Specific Protocols
WB protocol for GGH antibody 13264-1-APDownload protocol
IHC protocol for GGH antibody 13264-1-APDownload protocol
IP protocol for GGH antibody 13264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Front Pharmacol

Up-regulation of ABCG1 is associated with methotrexate resistance in acute lymphoblastic leukemia cells

Authors - Yao Chen
View Article
humanIHC,IF

Reprod Sci

The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning

Authors - Cheng Zhu
View Article