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验证数据展示

  • WB analysis of human placenta using 55038-1-AP

    WB analysis of human placenta using 55038-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 55038-1-AP (GFRA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 55038-1-AP

    IHC staining of human lung cancer using 55038-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55038-1-AP (GFRA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GFRA2 Polyclonal antibody

GFRA2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55038-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GDNF family receptor alpha 2, GDNF family receptor alpha-2, GDNF receptor alpha 2, GDNF receptor alpha-2, GDNF receptor beta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55038-1-AP targets GFRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GDNF family receptor alpha 2
别名 GDNF family receptor alpha 2, GDNF family receptor alpha-2, GDNF receptor alpha 2, GDNF receptor alpha-2, GDNF receptor beta
计算分子量 52 kDa
观测分子量 41-45 kDa
GenBank蛋白编号NM_001495
基因名称 GFRA2
Gene ID (NCBI) 2675
RRIDAB_10859940
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00451
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-52 kDa. This antibody is specific to GFRA2.

实验方案

Product Specific Protocols
IHC protocol for GFRA2 antibody 55038-1-APDownload protocol
WB protocol for GFRA2 antibody 55038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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