GFRA2 Polyclonal antibody

GFRA2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21973-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

GDNF family receptor alpha 2, GDNF family receptor alpha-2, GDNF receptor alpha 2, GDNF receptor alpha-2, GDNF receptor beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue, L02 cells, U-87 MG cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21973-1-AP targets GFRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原

CatNo: Ag16893

Product name: Recombinant human GFRA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 270-464 aa of BC041688

Sequence: DFHANCRASYQTVTSCPADNYQACLGSYAGMIGFDMTPNYVDSSPTGIVVSPWCSCRGSGNMEEECEKFLRDFTENPCLRNAIQAFGNGTDVNVSPKGPSFQATQAPRVEKTPSLPDDLSDSTSLGTSVITTCTSVQEQGLKANNSKELSMCFTELTTNIIPGSNKVIKPNSGPSRARPSAALTVLSVLMLKLAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GDNF family receptor alpha 2
别名 GDNF family receptor alpha 2, GDNF family receptor alpha-2, GDNF receptor alpha 2, GDNF receptor alpha-2, GDNF receptor beta
计算分子量 464 aa, 52 kDa
观测分子量 34-40 kDa, 50-55 kDa
GenBank蛋白编号BC041688
基因名称 GFRA2
Gene ID (NCBI) 2675
RRIDAB_11124728
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00451
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-55 kDa. This antibody recognizes all the three isoforms.

实验方案

Product Specific Protocols
WB protocol for GFRA2 antibody 21973-1-APDownload protocol
IHC protocol for GFRA2 antibody 21973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Lett

GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer.

Authors - Jiangning Gu
ratIHC

Sci Rep

Hypoxia adaptation mechanism in rats' peripheral auditory system in high altitude migration: a time series transcriptome analysis

Authors - Luoying Wang
ratWB

Biotechnol Appl Biochem

Ellagic acid supports neuron by regulating astroglia Nrf2.

Authors - Guo-Qing Wang
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