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验证数据展示

  • WB analysis using 14274-1-AP (same clone as 14274-1-PBS)

    WB analysis using 14274-1-AP (same clone as 14274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    IP experiment of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

    IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

GFM1 Polyclonal antibody, PBS Only

GFM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14274-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

COXPD1, EF Gmt, EFG1, EFGM, EF-Gmt

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14274-1-PBS targets GFM1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5616

Product name: Recombinant human GFM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-751 aa of BC049210

Sequence: RMHADMMEDVEEVYAGDICALFGIDCASGDTFTDKANSGLSMESIHVPDPVISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKETVISGMGELHLEIYAQRLEREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVEDYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G elongation factor, mitochondrial 1
别名 COXPD1, EF Gmt, EFG1, EFGM, EF-Gmt
计算分子量 86 kDa
观测分子量85 kDa
GenBank蛋白编号BC049210
基因名称 GFM1
Gene ID (NCBI) 85476
RRIDAB_2110140
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RP9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]

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