验证数据展示

WB analysis using 11293-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11293-1-AP (ALR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11293-1-AP
WB result of ALR antibody (11293-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALR transfected HepG2 cells.
IHC staining of human liver cancer using 11293-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11293-1-AP (ALR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11293-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11293-1-AP (ALR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of mouse liver using 11293-1-AP
IP result of anti-ALR (IP:11293-1-AP, 3ug; Detection:11293-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IF Staining of mouse kidney using 11293-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ALR antibody (11293-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab.
FC experiment of HepG2 using 11293-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ALR Polyclonal antibody (11293-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).
FC experiment of A431 using 11293-1-AP
1x10^6 A431 cells were intracellularly stained with 0.25 ug ALR Polyclonal antibody (11293-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

Featured Product
KD/KO验证

ALR Polyclonal antibody

ALR Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 11293-1-AP

产品说明书

引用文献(30)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

GFER, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR, Hepatopoietin

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (30)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse liver tissue, A431 cells, A375 cells, rat liver tissue, HepG2 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue, human testis tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue
Positive FC (Intra) detected in	A431 cells, HepG2 cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 8 publications below
WB	See 25 publications below
IHC	See 3 publications below
IF	See 9 publications below
IP	See 1 publications below

产品信息

11293-1-AP targets ALR in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag1840 Product name: Recombinant human GFER protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC028348 Sequence: MRTQQKRDTKFREDCPPDREELGRHSWAVLHTLAAYYPDLPTPEQQQDMAQFIHLFSKFYPCEECAEDLRKRLCRNHPDTRTRACFTQWLCHLHNEVNRKLGKPDFDCSKVDERWRDGWKDGSCD 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	growth factor, augmenter of liver regeneration
别名	GFER, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR, Hepatopoietin
计算分子量	15 kDa, 23 kDa
观测分子量	23-25 kDa
GenBank蛋白编号	BC028348
基因名称	GFER
Gene ID (NCBI)	2671
RRID	AB_2109970
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P55789
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

GFER (FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria. The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23/21 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus (PMID: 19409522, 24880092, 21152698).

实验方案

Product Specific Protocols
IF protocol for ALR antibody 11293-1-AP	Download protocol
IHC protocol for ALR antibody 11293-1-AP	Download protocol
IP protocol for ALR antibody 11293-1-AP	Download protocol
WB protocol for ALR antibody 11293-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell Stem Cell Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal. Authors - Rashim Pal Singh KD Validated View Article
mouse,human	WB	Hepatology Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation. Authors - Chao Zhang KD Validated View Article
human	WB,IF	Am J Hum Genet The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency. Authors - Di Fonzo Alessio A View Article
human	WB,IF	Aging Cell Dependence of PINK1 accumulation on mitochondrial redox system. Authors - Feng Gao View Article
mouse	WB	Elife Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8. Authors - Hsiang-Chun Chang KD Validated View Article
mouse	WB,IF	FASEB J Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression. Authors - Sudhir Kumar KO Validated View Article

验证数据展示

WB analysis using 11293-1-AP

WB analysis of HepG2 using 11293-1-AP

IHC staining of human liver cancer using 11293-1-AP

IHC staining of human liver cancer using 11293-1-AP

IP experiment of mouse liver using 11293-1-AP

IF Staining of mouse kidney using 11293-1-AP

FC experiment of HepG2 using 11293-1-AP

FC experiment of A431 using 11293-1-AP

ALR Polyclonal antibody

ALR Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation.

The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency.

Dependence of PINK1 accumulation on mitochondrial redox system.

Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8.

Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression.