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验证数据展示

  • IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

    IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

    IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

    IP experiment of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

    IP result of anti-GEMIN8 (IP:15251-1-AP, 3ug; Detection:15251-1-AP 1:400) with HepG2 cells lysate 1720ug. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

    IF Staining of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

  • WB analysis using 15251-1-AP (same clone as 15251-1-PBS)

    WB analysis using 15251-1-AP (same clone as 15251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

GEMIN8 Polyclonal antibody, PBS Only

GEMIN8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15251-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15251-1-PBS targets GEMIN8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6774

Product name: Recombinant human GEMIN8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-242 aa of BC003607

Sequence: MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 8
别名 Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1
计算分子量 29 kDa
观测分子量 32 kDa
GenBank蛋白编号BC003607
基因名称 GEMIN8
Gene ID (NCBI) 54960
RRIDAB_2111845
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWZ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.

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