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GEMIN8 Polyclonal antibody

GEMIN8 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15251-1-AP

Print datasheet

Synonyms

FAM51A1, Gem associated protein 8, Gemin 8, GEMIN8, Protein FAM51A1

验证数据展示

  • WB analysis of HepG2 using 15251-1-AP

    WB analysis of HepG2 using 15251-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15251-1-AP

    IHC staining of human liver cancer using 15251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 15251-1-AP

    IHC staining of human liver cancer using 15251-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HepG2 using 15251-1-AP

    IP experiment of HepG2 using 15251-1-AP

    IP result of anti-GEMIN8 (IP:15251-1-AP, 3ug; Detection:15251-1-AP 1:400) with HepG2 cells lysate 1720ug.

  • IF Staining of HepG2 using 15251-1-AP

    IF Staining of HepG2 using 15251-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15251-1-AP targets GEMIN8 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GEMIN8 fusion protein Ag6774 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gem (nuclear organelle) associated protein 8
Synonyms FAM51A1, Gem associated protein 8, Gemin 8, GEMIN8, Protein FAM51A1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC003607
Gene Symbol GEMIN8
Gene ID (NCBI) 54960
RRIDAB_2111845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWZ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.

实验方案

Product Specific Protocols
WB protocol for GEMIN8 antibody 15251-1-APDownload protocol
IHC protocol for GEMIN8 antibody 15251-1-APDownload protocol
IF protocol for GEMIN8 antibody 15251-1-APDownload protocol
IP protocol for GEMIN8 antibody 15251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Authors - Anne-Sophie Gribling-Burrer
View Article