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GEMIN6 Polyclonal antibody

GEMIN6 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12307-2-AP

Print datasheet

Synonyms

Gem associated protein 6, Gemin 6, GEMIN6, SIP2

验证数据展示

  • IHC staining of human breast cancer using 12307-2-AP

    IHC staining of human breast cancer using 12307-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12307-2-AP (GEMIN6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 12307-2-AP

    IHC staining of human breast cancer using 12307-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12307-2-AP (GEMIN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 12307-2-AP

    WB analysis of HEK-293 using 12307-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12307-2-AP (GEMIN6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 12307-2-AP

    IF Staining of PC-3 using 12307-2-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 12307-2-AP (GEMIN6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12307-2-AP targets GEMIN6 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GEMIN6 fusion protein Ag2954 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gem (nuclear organelle) associated protein 6
Synonyms Gem associated protein 6, Gemin 6, GEMIN6, SIP2
Calculated Molecular Weight 167 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC018195
Gene Symbol GEMIN6
Gene ID (NCBI) 79833
RRIDAB_2111835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEMIN6, also named as SIP2, is a subunit of SMN complex. It plays an important role in the splicing of cellular pre-mRNAs.

实验方案

Product Specific Protocols
WB protocol for GEMIN6 antibody 12307-2-APDownload protocol
IHC protocol for GEMIN6 antibody 12307-2-APDownload protocol
IF protocol for GEMIN6 antibody 12307-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Authors - Anne-Sophie Gribling-Burrer
View Article
humanWB

Nucleic Acids Res

NUFIP and the HSP90/R2TP chaperone bind the SMN complex and facilitate assembly of U4-specific proteins.

Authors - Jonathan Bizarro
View Article