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验证数据展示

  • WB analysis of HEK-293 using 12408-1-AP (same clone as 12408-1-PBS)

    WB analysis of HEK-293 using 12408-1-AP (same clone as 12408-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12408-1-AP (GEMIN4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12408-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 cells using 12408-1-AP (same clone as 12408-1-PBS)

    IP experiment of HEK-293 cells using 12408-1-AP (same clone as 12408-1-PBS)

    IP result of anti-GEMIN4 (12408-1-AP for IP and Detection) with 293 cell lysate. This data was developed using the same antibody clone with 12408-1-PBS in a different storage buffer formulation.

GEMIN4 Polyclonal antibody, PBS Only

GEMIN4 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12408-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12408-1-PBS targets GEMIN4 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3099

Product name: Recombinant human GEMIN4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 703-1058 aa of BC020062

Sequence: QLLDRFSKYWQLPKEKRCLSLDRKDLAIHILELLCEIVSANAETFSPDVWIKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGAGTGLLAWMECCCVSSGISERMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQRLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLFLRTFQFLCSHSCRNWLPLEGWNHVVKLLCGSLTRLLDSVRAIQAAGPWVQGPEQDLTQEALFVYTQVFCHALHIMAMLHPEVCEPLYVLSIETLTCYETLSKTNPSVSSLRSRAHEQRFLKSIAEGIGPEERRQTLLQKMSSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 4
别名
计算分子量 1058 aa, 120 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC020062
基因名称 GEMIN4
Gene ID (NCBI) 50628
RRIDAB_10640891
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57678
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GEMIN4, also named as P97, is a sub-unit of SMN complex. The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs). It plays an important role in the splicing of cellular pre-mRNAs. The express amount of GEMIN4 is about 8.79 ppm (http://pax-db.org/#!protein/984742). This antibody detects 97-110 kDa GEMIN4.

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