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验证数据展示

  • WB analysis of HEK-293 using 12408-1-AP

    WB analysis of HEK-293 using 12408-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12408-1-AP (GEMIN4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 cells using 12408-1-AP

    IP experiment of HEK-293 cells using 12408-1-AP

    IP result of anti-GEMIN4 (12408-1-AP for IP and Detection) with 293 cell lysate.

GEMIN4 Polyclonal antibody

GEMIN4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12408-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

GEMIN4, HC56, HCAP1, HHRF 1, p97

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12408-1-AP targets GEMIN4 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3099

Product name: Recombinant human GEMIN4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 703-1058 aa of BC020062

Sequence: QLLDRFSKYWQLPKEKRCLSLDRKDLAIHILELLCEIVSANAETFSPDVWIKSLSWLHRKLEQLDWTVGLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGAGTGLLAWMECCCVSSGISERMLSLLVVDVGNPEEVRLFSKGFLVALVQVMPWCSPQEWQRLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLFLRTFQFLCSHSCRNWLPLEGWNHVVKLLCGSLTRLLDSVRAIQAAGPWVQGPEQDLTQEALFVYTQVFCHALHIMAMLHPEVCEPLYVLSIETLTCYETLSKTNPSVSSLRSRAHEQRFLKSIAEGIGPEERRQTLLQKMSSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gem (nuclear organelle) associated protein 4
别名 GEMIN4, HC56, HCAP1, HHRF 1, p97
计算分子量 1058 aa, 120 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC020062
基因名称 GEMIN4
Gene ID (NCBI) 50628
RRIDAB_10640891
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GEMIN4, also named as P97, is a sub-unit of SMN complex. The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs). It plays an important role in the splicing of cellular pre-mRNAs. The express amount of GEMIN4 is about 8.79 ppm (http://pax-db.org/#!protein/984742). This antibody detects 97-110 kDa GEMIN4.

实验方案

Product Specific Protocols
IP protocol for GEMIN4 antibody 12408-1-APDownload protocol
WB protocol for GEMIN4 antibody 12408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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