验证数据展示
经过测试的应用
| Positive WB detected in | pig bone marrow tissue, ROS1728 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66371-1-Ig targets GDF10 in WB, ELISA applications and shows reactivity with human, pig, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, pig, rat |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag4906 Product name: Recombinant human GDF10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-288 aa of BC028237 Sequence: MALAPPPRGLWQAKDISPIVKAARRDGELLLSAQLDSEERDPGVPRPSPYAPYILVYANDLAISEPNSVAVTLQRYDPFPAGDPEPRAAPNNSADPRVRRAAQATGPLQDNELPGLDERPPRAHAQHFHKHQLWPSPFRALKPRPGRKDRRKKGQEVFMAASQVLDFDEKTMQKARRKQWDEPRVCSRRYLKVDFADIGWNEWIISPKSFDAYYCAGACEFPMPKIVRPSNHATIQSIVRAVGIIPGIPEPCCVPDKMNSLGVLFLDENRNVVLKVYPNMSVDTCACR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | growth differentiation factor 10 |
| 别名 | BIP, BMP 3b, BMP3B, Bone inducing protein, Bone morphogenetic protein 3B, GDF 10, GDF10 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC028237 |
| 基因名称 | GDF10 |
| Gene ID (NCBI) | 2662 |
| RRID | AB_2881750 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P55107 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Growth differentiation factor-10 (GDF10), also known as BMP3b, is a member of the transforming growth factor-b (TGF-b) superfamily. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This promotes neural repair after stroke. GDF10 plays a role in head formation and may have multiple roles in skeletal morphogenesis. Additionally, GDF10 may act as a tumor suppressor and reduced expression of this gene is associated with oral cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GDF10 antibody 66371-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
