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验证数据展示

  • WB analysis of HEK-293 using 16726-1-AP (same clone as 16726-1-PBS)

    WB analysis of HEK-293 using 16726-1-AP (same clone as 16726-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 16726-1-AP (same clone as 16726-1-PBS)

    IF Staining of MCF-7 using 16726-1-AP (same clone as 16726-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using GCSH antibody 16726-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 16726-1-AP (same clone as 16726-1-PBS)

    IP experiment of mouse kidney using 16726-1-AP (same clone as 16726-1-PBS)

    IP result of anti-GCSH (IP:16726-1-AP, 3ug; Detection:16726-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 16726-1-AP (same clone as 16726-1-PBS)

    IHC staining of human ovary cancer using 16726-1-AP (same clone as 16726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16726-1-AP (GCSH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GCSH Polyclonal antibody, PBS Only

GCSH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16726-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16726-1-PBS targets GCSH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10174

Product name: Recombinant human GCSH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-173 aa of BC000790

Sequence: MALRVVRSVRALLCTLRAVPLPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycine cleavage system protein H (aminomethyl carrier)
别名 NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE
计算分子量 19 kDa
观测分子量 15 kDa
GenBank蛋白编号BC000790
基因名称 GCSH
Gene ID (NCBI) 2653
RRIDAB_2247351
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23434
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

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