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验证数据展示

  • WB analysis using 30693-1-AP (same clone as 30693-1-PBS)

    WB analysis using 30693-1-AP (same clone as 30693-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30693-1-AP (ACRC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30693-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 30693-1-AP (same clone as 30693-1-PBS)

    IHC staining of human testis using 30693-1-AP (same clone as 30693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 30693-1-AP (GCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30693-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 30693-1-AP (same clone as 30693-1-PBS)

    IHC staining of human testis using 30693-1-AP (same clone as 30693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 30693-1-AP (GCNA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30693-1-PBS in a different storage buffer formulation.

GCNA Polyclonal antibody, PBS Only

GCNA Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30693-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

ACRC, Acidic repeat-containing protein, Germ cell nuclear acidic peptidase, Germ cell nuclear acidic protein, Germ cell nuclear antigen

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30693-1-PBS targets GCNA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33720

Product name: Recombinant human ACRC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-137 aa of BC136259

Sequence: MDGCKKELPRLQEPEEDEDCYILNVQSSSDDTSGSSVARRAPKRQASCILNVQSRSGDTSGSSVARRAPKRQASSVVVIDSDSDEECHTHEEKKAKLLEINSDDESPECCHVKPAIQEPPIVISDDDNDDDNGNDLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acidic repeat containing
别名 ACRC, Acidic repeat-containing protein, Germ cell nuclear acidic peptidase, Germ cell nuclear acidic protein, Germ cell nuclear antigen
计算分子量 691 aa, 76 kDa
观测分子量76 kDa
GenBank蛋白编号BC136259
基因名称 GCNA
Gene ID (NCBI) 93953
RRIDAB_3086389
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QF7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Germ cell nuclear acidic protein (GCNA) has been extensively used as a germ cell marker for almost 30 years. Recently, mutations in GCNA have been linked to azoospermia in humans, defining GCNA as a clinical determinant of human infertility.

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