验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, PC-3 cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30693-1-AP targets GCNA in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag33720 Product name: Recombinant human ACRC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-137 aa of BC136259 Sequence: MDGCKKELPRLQEPEEDEDCYILNVQSSSDDTSGSSVARRAPKRQASCILNVQSRSGDTSGSSVARRAPKRQASSVVVIDSDSDEECHTHEEKKAKLLEINSDDESPECCHVKPAIQEPPIVISDDDNDDDNGNDLE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acidic repeat containing |
| 别名 | acidic repeat containing, ACRC, GCNA, NAAR1 |
| 计算分子量 | 691 aa, 76 kDa |
| 观测分子量 | 76 kDa |
| GenBank蛋白编号 | BC136259 |
| 基因名称 | GCNA |
| Gene ID (NCBI) | 93953 |
| RRID | AB_3086389 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96QF7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Germ cell nuclear acidic protein (GCNA) has been extensively used as a germ cell marker for almost 30 years. Recently, mutations in GCNA have been linked to azoospermia in humans, defining GCNA as a clinical determinant of human infertility.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GCNA antibody 30693-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
