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验证数据展示

  • WB analysis using 61029-1-Ig (same clone as 61029-1-PBS)

    WB analysis using 61029-1-Ig (same clone as 61029-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 61029-1-Ig (GBX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 61029-1-Ig (same clone as 61029-1-PBS)

    WB analysis of PC-3 using 61029-1-Ig (same clone as 61029-1-PBS)

    WB result of GBX2 antibody (61029-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected PC-3 cells. This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 61029-1-Ig (same clone as 61029-1-PBS)

    IF Staining of HeLa using 61029-1-Ig (same clone as 61029-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (61029-1-Ig, Clone: 4F3E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 61029-1-Ig (same clone as 61029-1-PBS)

    IHC staining of mouse brain using 61029-1-Ig (same clone as 61029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 61029-1-Ig (GBX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 61029-1-PBS in a different storage buffer formulation.

GBX2 Monoclonal antibody, PBS Only

GBX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 61029-1-PBS

产品说明书

CloneNo. 4F3E4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Gastrulation and brain-specific homeobox protein 2, Homeobox protein GBX-2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

61029-1-PBS targets GBX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17109

Product name: Recombinant human GBX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 65-248 aa of BC137448

Sequence: LPQAALQPALPPAHPHHQIPSLPTGFCSSLAQGMALTSTLMATLPGGFSASPQHQEAAAARKFAPQPLPGGGNFDKAEALQADAEDGKGFLAKEGSLLAFSAAETVQASLVGAVRGQGKDESKVEDDPKGKEESFSLESDVDYSSDDNLTGQAAHKEEDPGHALEETPPSSGAAGSTTSTGKNR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 gastrulation brain homeobox 2
别名 Gastrulation and brain-specific homeobox protein 2, Homeobox protein GBX-2
计算分子量 348 aa, 37 kDa
观测分子量37 kDa
GenBank蛋白编号BC137448
基因名称 GBX2
Gene ID (NCBI) 2637
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP52951
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Gastrulation Brain Homeobox 2 (GBX2) is a transcription factor belonging to the homeobox gene family, which plays a critical role in embryonic development, particularly in brain patterning and neural differentiation. It is highly conserved across vertebrates and is essential for the proper formation of the midbrain-hindbrain boundary and the development of the cerebellum. GBX2 is expressed in specific regions of the developing central nervous system and regulates downstream target genes involved in cell fate determination and tissue morphogenesis. Dysregulation or mutations in GBX2 have been associated with developmental disorders and may contribute to tumorigenesis in certain cancers. (PMID: 11063941; PMID: 31758726)

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