验证数据展示
经过测试的应用
| Positive WB detected in | NCI-H1299 cells, C2C12 cells, PC-3 cells, HCT 116 cells, A172 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
61029-1-Ig targets GBX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17109 Product name: Recombinant human GBX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 65-248 aa of BC137448 Sequence: LPQAALQPALPPAHPHHQIPSLPTGFCSSLAQGMALTSTLMATLPGGFSASPQHQEAAAARKFAPQPLPGGGNFDKAEALQADAEDGKGFLAKEGSLLAFSAAETVQASLVGAVRGQGKDESKVEDDPKGKEESFSLESDVDYSSDDNLTGQAAHKEEDPGHALEETPPSSGAAGSTTSTGKNR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | gastrulation brain homeobox 2 |
| 别名 | Gastrulation and brain-specific homeobox protein 2, Homeobox protein GBX-2 |
| 计算分子量 | 348 aa, 37 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC137448 |
| 基因名称 | GBX2 |
| Gene ID (NCBI) | 2637 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P52951 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gastrulation Brain Homeobox 2 (GBX2) is a transcription factor belonging to the homeobox gene family, which plays a critical role in embryonic development, particularly in brain patterning and neural differentiation. It is highly conserved across vertebrates and is essential for the proper formation of the midbrain-hindbrain boundary and the development of the cerebellum. GBX2 is expressed in specific regions of the developing central nervous system and regulates downstream target genes involved in cell fate determination and tissue morphogenesis. Dysregulation or mutations in GBX2 have been associated with developmental disorders and may contribute to tumorigenesis in certain cancers. (PMID: 11063941; PMID: 31758726)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GBX2 antibody 61029-1-Ig | Download protocol |
| IHC protocol for GBX2 antibody 61029-1-Ig | Download protocol |
| WB protocol for GBX2 antibody 61029-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
