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GBX2 Polyclonal antibody

GBX2 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21639-1-AP

Print datasheet

Synonyms

gastrulation brain homeobox 2, GBX2, Homeobox protein GBX 2

验证数据展示

  • WB analysis of HeLa using 21639-1-AP

    WB analysis of HeLa using 21639-1-AP

    WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells.

  • WB analysis of mouse stomach using 21639-1-AP

    WB analysis of mouse stomach using 21639-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 21639-1-AP

    IP experiment of HeLa using 21639-1-AP

    IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug.

  • IF Staining of HeLa using 21639-1-AP

    IF Staining of HeLa using 21639-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21639-1-AP (GBX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse stomach tissue
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21639-1-AP targets GBX2 in WB, IP, IF, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, chIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen GBX2 fusion protein Ag16288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gastrulation brain homeobox 2
Synonyms gastrulation brain homeobox 2, GBX2, Homeobox protein GBX 2
Calculated Molecular Weight 348 aa, 37 kDa
Observed Molecular Weight 33-37 kDa
GenBank Accession NumberBC137448
Gene Symbol GBX2
Gene ID (NCBI) 2637
RRIDAB_2878896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52951
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

实验方案

Product Specific Protocols
WB protocol for GBX2 antibody 21639-1-APDownload protocol
IF protocol for GBX2 antibody 21639-1-APDownload protocol
IP protocol for GBX2 antibody 21639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

RNA Biol

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Authors - Xiwen Li
View Article
humanWB,ChIP

Funct Integr Genomics

Oncogenic GBX2 promotes the malignant behaviors of bladder cancer cells by binding to the ITGA5 promoter and activating its transcription.

Authors - Yaoyao Xiong
View Article