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验证数据展示

  • WB analysis of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    WB analysis of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    IF Staining of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (21639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse stomach using 21639-1-AP (same clone as 21639-1-PBS)

    WB analysis of mouse stomach using 21639-1-AP (same clone as 21639-1-PBS)

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    IP experiment of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GBX2 Polyclonal antibody, PBS Only

GBX2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 21639-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

Gastrulation and brain-specific homeobox protein 2, Homeobox protein GBX-2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

21639-1-PBS targets GBX2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16288

Product name: Recombinant human GBX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 65-248 aa of BC137448

Sequence: LPQAALQPALPPAHPHHQIPSLPTGFCSSLAQGMALTSTLMATLPGGFSASPQHQEAAAARKFAPQPLPGGGNFDKAEALQADAEDGKGFLAKEGSLLAFSAAETVQASLVGAVRGQGKDESKVEDDPKGKEESFSLESDVDYSSDDNLTGQAAHKEEDPGHALEETPPSSGAAGSTTSTGKNR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gastrulation brain homeobox 2
别名 Gastrulation and brain-specific homeobox protein 2, Homeobox protein GBX-2
计算分子量 348 aa, 37 kDa
观测分子量 33-37 kDa
GenBank蛋白编号BC137448
基因名称 GBX2
Gene ID (NCBI) 2637
RRIDAB_2878896
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52951
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

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