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验证数据展示

  • FC experiment of HepG2 using CL647-11854

    FC experiment of HepG2 using CL647-11854

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GBP2 Polyclonal antibody (CL647-11854)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL647-11854

    IF Staining of HeLa using CL647-11854

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 GBP2 antibody (CL647-11854) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-11854

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Guanylate-binding protein 2, Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11854-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11854 targets GBP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 GBP2 fusion protein Ag2425 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanylate binding protein 2, interferon-inducible
别名 Guanylate-binding protein 2, Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2
计算分子量 591 aa, 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC022272
基因名称 GBP2
Gene ID (NCBI) 2634
RRIDAB_2923951
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32456
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 GBP2 antibody CL647-11854Download protocol
Standard Protocols
Click here to view our Standard Protocols