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验证数据展示

  • WB analysis using 84264-6-RR

    WB analysis using 84264-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84264-6-RR (GBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84264-6-RR

    IHC staining of human tonsillitis using 84264-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84264-6-RR (GBP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84264-6-RR

    IF Staining of HeLa using 84264-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (84264-6-RR, Clone: 241580C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 84264-6-RR

    FC experiment of A549 using 84264-6-RR

    1x10^6 A549 cells were intracellularly stained with 0.2 ug GBP2 Recombinant antibody (84264-6-RR, Clone:241580C12) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84264-6-RR

    Affinity and Kinetic Characterization of 84264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84264-6-RR against Human GBP2 were performed. The affinity constant is below 1 pM.

GBP2 Recombinant antibody

GBP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84264-6-RR

产品说明书

CloneNo. 241580C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

241580C12, EC:3.6.5.-, GBP 2, GBP-2, GTP-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, IM-9 cells, HaCaT cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84264-6-RR targets GBP2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GBP2 fusion protein Ag2425 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanylate binding protein 2, interferon-inducible
别名 241580C12, EC:3.6.5.-, GBP 2, GBP-2, GTP-binding protein 2
计算分子量 591 aa, 67 kDa
观测分子量67 kDa
GenBank蛋白编号BC022272
基因名称 GBP2
Gene ID (NCBI) 2634
RRIDAB_3671810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP32456
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

实验方案

Product Specific Protocols
WB protocol for GBP2 antibody 84264-6-RRDownload protocol
IHC protocol for GBP2 antibody 84264-6-RRDownload protocol
IF protocol for GBP2 antibody 84264-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols