GBP2 Recombinant antibody

GBP2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84264-6-RR

产品说明书

CloneNo. 241580C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, IM-9 cells, HaCaT cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84264-6-RR targets GBP2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GBP2 fusion protein Ag2425 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanylate binding protein 2, interferon-inducible
别名 Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2, EC:3.6.5.-
计算分子量 591 aa, 67 kDa
观测分子量67 kDa
GenBank蛋白编号BC022272
基因名称 GBP2
Gene ID (NCBI) 2634
RRIDAB_3671810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP32456
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

实验方案

Product Specific Protocols
WB protocol for GBP2 antibody 84264-6-RRDownload protocol
IF protocol for GBP2 antibody 84264-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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