验证数据展示
经过测试的应用
Positive WB detected in | HT-1376 cells, Saos-2 cells, HepG2 cells, PC-3 cells, A375 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68559-1-Ig targets GBP2 in WB, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human |
免疫原 | GBP2 fusion protein Ag24108 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | guanylate binding protein 2, interferon-inducible |
别名 | GBP 2, GBP2, GTP binding protein 2, HuGBP 2 |
计算分子量 | 591 aa, 67 kDa |
观测分子量 | 67 kDa |
GenBank蛋白编号 | BC022272 |
基因名称 | GBP2 |
Gene ID (NCBI) | 2634 |
RRID | AB_3085261 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P32456 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)
实验方案
Product Specific Protocols | |
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WB protocol for GBP2 antibody 68559-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |