验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, OVCAR-3 cells, SKOV-3 cells, mouse ovary tissue, rat ovary tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32983-1-AP targets GBGT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag38506 Product name: Recombinant human GBGT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 185-347 aa of NM_021996.5 Sequence: ETISQHIAKRAHREVDYLFCLDVDMVFRNPWGPETLGDLVAAIHPSYYAVPRQQFPYERRRVSTAFVADSEGDFYYGGAVFGGQVARVYEFTRGCHMAILADKANGIMAAWREESHLNRHFISNKPSKVLSPEYLWDDRKPQPPSLKLIRFSTLDKDISCLRS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 |
| 别名 | Forssman glycolipid synthase-like protein, Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1, UNQ2513/PRO6002 |
| 计算分子量 | 40kDa，347aa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | NM_021996.5 |
| 基因名称 | GBGT1 |
| Gene ID (NCBI) | 26301 |
| RRID | AB_3742812 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N5D6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GBGT1 (Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1) gene is located on chromosome 9 (9q34) and comprises 347 amino acids. GBGT1 is the most differentially expressed glycogene involved in the biosynthesis of Fs antigen and GBGT1 is differentially methylated and associated with inflammatory bowel disease (PMID: 25294702). GBGT1 mRNA expression has been observed in a variety of human tissues being highest in placenta and ovary (PMID: 31933576).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GBGT1 antibody 32983-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
