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  • Featured Product
  • KD/KO Validated

GBF1 Polyclonal antibody

GBF1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25183-1-AP

Print datasheet

Synonyms

ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248

验证数据展示

  • IHC staining of human prostate cancer using 25183-1-AP

    IHC staining of human prostate cancer using 25183-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP (GBF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 25183-1-AP

    IHC staining of human prostate cancer using 25183-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP (GBF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 25183-1-AP

    WB analysis of HeLa using 25183-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25183-1-AP (GBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25183-1-AP

    IF Staining of HeLa using 25183-1-AP

    Immunofluorescent analysis of HeLa cells using 25183-1-AP (GBF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25183-1-AP is GBF1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GBF1 fusion protein Ag16579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1
Synonyms ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248
Calculated Molecular Weight 1859 aa, 206 kDa
Observed Molecular Weight 206 kDa
GenBank Accession NumberBC117681
Gene Symbol GBF1
Gene ID (NCBI) 8729
RRIDAB_2879948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GBF1 antibody 25183-1-APDownload protocol
IHC protocol for GBF1 antibody 25183-1-APDownload protocol
IF protocol for GBF1 antibody 25183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang
View Article
humanWB

Sci Rep

The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.

Authors - Xiaobo Lei
  • KD Validated
View Article