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验证数据展示

  • WB analysis using 17164-1-AP

    WB analysis using 17164-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17164-1-AP (GBA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17164-1-AP

    WB analysis using 17164-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 17164-1-AP (GBA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GBA2 Polyclonal antibody

GBA2 Polyclonal Antibody for WB, ELISA
Cat No. 17164-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Bile acid beta-glucosidase GBA2, Beta-glucosidase 2, Beta-glucocerebrosidase 2, Beta glucosidase 2, Beta glucocerebrosidase 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, HeLa cells, mouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17164-1-AP targets GBA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10606

Product name: Recombinant human GBA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 574-927 aa of BC011363

Sequence: RRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEGCYRTVWERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQQHKKASWPKVKQGTGLRTGPMFGPKEAMANLSPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucosidase, beta (bile acid) 2
别名 Bile acid beta-glucosidase GBA2, Beta-glucosidase 2, Beta-glucocerebrosidase 2, Beta glucosidase 2, Beta glucocerebrosidase 2
计算分子量 927 aa, 105 kDa
观测分子量 105 kDa
GenBank蛋白编号BC011363
基因名称 GBA2
Gene ID (NCBI) 57704
RRIDAB_2109077
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCG7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GBA2(Non-lysosomal glucosylceramidase) is also named as KIAA1605,NLGase and belongs to the non-lysosomal glucosylceramidase family.It catalyzes the conversion of glucosylceramide to free glucose and ceramide and is involved in sphingomyelin generation and prevention of glycolipid accumulation.GBA2 contains a putative membrane-spanning domain and 2 potential N-glycosylation sites. However, glycosidase treatment indicated that GBA2 is not a glycoprotein(PMID:11489889).It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for GBA2 antibody 17164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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