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验证数据展示

  • WB analysis using 84987-3-RR

    WB analysis using 84987-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84987-3-RR (GATA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84987-3-RR

    IF Staining of HeLa using 84987-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GATA6 antibody (84987-3-RR, Clone: 242325B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 84987-3-RR

    FC experiment of HEK-293 using 84987-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATA6 Recombinant antibody (84987-3-RR, Clone:242325B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

GATA6 Recombinant antibody

GATA6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84987-3-RR

产品说明书

CloneNo. 242325B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6, 242325B6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, MCF-7 cells, HepG2 cells, SGC-7901 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84987-3-RR targets GATA6 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GATA binding protein 6
别名 Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6, 242325B6
计算分子量 60 kDa
观测分子量 60-64 kDa
GenBank蛋白编号NM_005257
基因名称 GATA6
Gene ID (NCBI) 2627
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92908
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA6 is a member of the GATA family of zinc-finger transcriptional regulators, which play crucial roles in the regulation of cell growth, differentiation, survival, and maintenance of body functions. Named after the conserved base sequence (G/A)GATA(A/T), GATA6 features conserved tandem zinc fingers and is essential in coordinating the development and precise gene regulation of diverse tissues, including the heart and gastrointestinal tract. GATA6 recognizes the sequence (A/T/C)GATA(A/T)A and interacts with other transcriptional regulators through its zinc-finger domain.

实验方案

Product Specific Protocols
WB protocol for GATA6 antibody 84987-3-RRDownload protocol
IF protocol for GATA6 antibody 84987-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols