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验证数据展示

  • WB analysis using 85060-2-RR

    WB analysis using 85060-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85060-2-RR (SLC6A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 85060-2-RR

    IHC staining of mouse cerebellum using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 85060-2-RR

    IHC staining of mouse cerebellum using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 85060-2-RR

    IHC staining of rat brain using 85060-2-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85060-2-RR (GAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 85060-2-RR

    IF Staining of rat brain using 85060-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GAT1 antibody (85060-2-RR, Clone: 242637D11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85060-2-RR

    Affinity and Kinetic Characterization of 85060-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85060-2-RR against Human GAT1 were performed. The affinity constant is below 1 pM.

GAT1 Recombinant antibody

GAT1 Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85060-2-RR

产品说明书

CloneNo. 242637D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

SLC6A1, 242637D11, GABA Transporter 1, GABATHG, GABATR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inunboiled rat brain tissue
Positive IHC detected inmouse cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85060-2-RR targets GAT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GAT1 fusion protein Ag29712 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, GABA), member 1
别名 SLC6A1, 242637D11, GABA Transporter 1, GABATHG, GABATR
计算分子量 67 kDa
观测分子量67 kDa
GenBank蛋白编号BC033904
基因名称 GABA Transporter 1/GAT1
Gene ID (NCBI) 6529
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30531
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GAT1 antibody 85060-2-RRDownload protocol
IHC protocol for GAT1 antibody 85060-2-RRDownload protocol
IF protocol for GAT1 antibody 85060-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols