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验证数据展示

  • WB analysis using 67939-1-Ig

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67939-1-Ig

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67939-1-Ig

    IF Staining of HeLa using 67939-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GART antibody (67939-1-Ig, Clone: 2A11E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 67939-1-Ig

    FC experiment of HeLa using 67939-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GART Monoclonal antibody (67939-1-Ig, Clone:2A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

GART Monoclonal antibody

GART Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67939-1-Ig

产品说明书

CloneNo. 2A11E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67939-1-Ig targets GART in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30509

Product name: Recombinant human GART protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-318 aa of BC038958

Sequence: MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
别名 5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS
计算分子量 1010 aa, 108 kDa
观测分子量 46 kDa, 108 kDa
GenBank蛋白编号BC038958
基因名称 GART
Gene ID (NCBI) 2618
RRIDAB_2918691
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP22102
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GART is a trifunctional polypeptide that has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, and phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.

实验方案

Product Specific Protocols
FC protocol for GART antibody 67939-1-IgDownload protocol
IF protocol for GART antibody 67939-1-IgDownload protocol
WB protocol for GART antibody 67939-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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