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验证数据展示

  • IF Staining of HeLa using CL594-67939

    IF Staining of HeLa using CL594-67939

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 GART antibody (CL594-67939, Clone: 2A11E2 ) at dilution of 1:200.

CoraLite®594-conjugated GART Monoclonal antibody

GART Monoclonal Antibody for IF/ICC
Cat No. CL594-67939

产品说明书

CloneNo. 2A11E2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67939-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67939 targets GART in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 GART fusion protein Ag30509 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
别名 5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS
计算分子量 1010 aa, 108 kDa
观测分子量 46 kDa, 108 kDa
GenBank蛋白编号BC038958
基因名称 GART
Gene ID (NCBI) 2618
RRIDAB_2934787
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP22102
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL594 GART antibody CL594-67939Download protocol
Standard Protocols
Click here to view our Standard Protocols