验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67939-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67939 targets GART in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | GART fusion protein Ag30509 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase |
| 别名 | 5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS |
| 计算分子量 | 1010 aa, 108 kDa |
| 观测分子量 | 46 kDa, 108 kDa |
| GenBank蛋白编号 | BC038958 |
| 基因名称 | GART |
| Gene ID (NCBI) | 2618 |
| RRID | AB_2934787 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P22102 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GART antibody CL594-67939 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |