验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
13962-1-AP targets GALNT7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4917 Product name: Recombinant human GALNT7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 308-657 aa of BC047468 Sequence: VNWYAPLVAPISKDRTICTVPLIDVINGNTYEIIPQGGGDEDGYARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLFAIEREFFFELGLYDPGLQIWGGENFEISYKIWQCGGKLLFVPCSRVGHIYRLEGWQGNPPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQALPYGDISELKKFREDHNCKSFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELGPCHRMGGNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGKCLDRSEVLHQVFISNCDSSKTTQKWEMNNIHSV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) |
| 别名 | EC:2.4.1.41, GALNAC T7, GalNAcT7, GalNAc-T7, N-acetylgalactosaminyltransferase 7 |
| 计算分子量 | 75.4 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC047468 |
| 基因名称 | GALNT7 |
| Gene ID (NCBI) | 51809 |
| RRID | AB_2109009 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86SF2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GALNT7 is a member of GALNT family that are glycotransferases involved in oligosaccharide biosynthesis. Aberrant glycosylation has been linked to tumor development and progression. Increased expression of GALNT7 has been observed in cervical cancer cells. (PMID: 22399294)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GALNT7 antibody 13962-1-AP | Download protocol |
| WB protocol for GALNT7 antibody 13962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Transl Res Resveratrol enhances MUC2 synthesis via the ANRIL-miR-34a axis to mitigate IBD | ||
Eur J Histochem Histological and histochemical characterization of the musk gland in forest musk deer (Moschus berezovskii): a preliminary study |
